Effect of Shear Stress on Permeability of Vascular Endothelial Monolayer Cocultured with Smooth Muscle Cells(<Special Issue>Bioengineering)
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概要
- 論文の詳細を見る
Effect of fluid shear stress on permeability of endothelial monolayer was investigated using an endothelial cell (EC)-smooth muscle cell (SMC) cocultured model (CM). Permeability of ECs to bovine serum albumin was measured after exposure to shear stress of 1.5Pa for 48 hours. Morphology and VE-cadherin expression of ECs in CM was almost same as of ECs cultured alone (monocultured model, MM). Under static condition, EC permeability was 5.1±3.0×10^<-6>cm/sec (mean±SD) in MM and 6.5±3.4×10^<-6>cm/sec in CM. After exposure to shear stress, EC permeability in CM (2.2±1.9×10^<-6>cm/sec, p<0.05) significantly decreased compared with the static model. However, EC permeability in MM (3.9±3.2×10^<-6>cm/sec) did not significantly change compared with static cultured condition. These results suggested that cellular interactions between ECs and SMCs have important influences on EC permeability.
- 一般社団法人日本機械学会の論文
- 2004-12-15
著者
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SATO Masaaki
Biomechanics Laboratory, Graduate School of Mechanical Engineering, Tohoku University
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Sakamoto Naoya
Dept. Of Bioeng. & Robotics Tohoku Univ.
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SAKAMOTO Naoya
Biomechanics Laboratory, Department of Bioengineering and Robotics, Graduate School of Engineering,
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OHASHI Toshiro
Biomechanics Laboratory, Department of Bioengineering and Robotics, Graduate School of Engineering,
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Sato Masaaki
Biomechanics Laboratory Graduate School Of Mechanical Engineering Tohoku University
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