Studies on the Viruses of Solanaceous Vegetables in Japan. II. A Strain of Tobacco Mosaic Virus Isolated from Pepper Plants

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概要

• 論文の詳細を見る

• A strain of tobacco mosaic virus (TMV) was isolated from pepper (Capsicum annuum L. cv. Wakayamashishi) collected in Wakayama and Nara prefectures in October, 1970. The diseased pepper plants showed necrosis with some mild mottlings on the leaflets but most frequently on the stems. Symptomatology and host range of the virus were studied with 38 of plants species in 8 families. When the inoculated plants were held at 25-28 C, the virus causes systemic infection showing no symptom in Turkish tabacco (N. tobacum cvs. Xanthi and Samsun). In a few plants, one or two of the nearly mature leaves showed very mild green mottle symptoms, but subsequently developed leaves on these plants were free of sign although they contain the virus. In tobacco (N. tobacum cvs. Bright Yellow and White Burley) and N. sylvestris, the virus induced small brownish local lesions. Phaseolus vulgaris cv. Kairyo Otebo remained symptomless after inoculation with the virus and its recovery to N. glutinosa failed with extracts from the inoculated and the uninoculted tops. Tomato (Lycopersicon esculentum cv. Ponterosa, Marglove and Ohgata Fukujyu) remained symptomless after inoculation with the virus. But the virus was recovered from the inoculated leaves 23 days after inoculation. The virus differs from the other known strains of TMV described in Japan, due to the reaction of the above-mentioned plants. The thermal inactivation point of crude juice extract from infected Turkish tabacco leaves was 65-70 C for 10 minutes. The dilution end point was around 10^<-5>-10^<-6>. The virus was found to be more sensitive to UV irradiation than TMV-OM. In fact, after a dose of 1 minute, less than one percent of the initial virus activity of the strain was recovered. The virus used and TMV-OM showed the same single symmetrical schlieren peak. The mobility of the virus by SDS-polyacrylamide gel electrophoresis was also identical with TMV-OM. Serological positive reaction was observed between the virus and the TMV-OM antiserum in precipitin and agar gel diffusion tests. But cross protection against TMV-OM was imcomplete. Considering other properties of the virus, these results seem to indicate that the present virus stands in rather distant relationship to ordinary group of TMV. It appears from the results of tests in plants, and especially in Turkish tobacco and tomato that the present strain probably appropriates to the latent strain originally described by McKINNEY. Electron microscope study was made of the field pepper plants systemically infected with the present virus. An unusual kind of inclusions which are made up largely masses of angle layer aggregates were found in the sections of epidermis. The same aggregates were also observed in the local lesion area of Gomphrena globosa and Tetragonia expansa.

• 大阪府立大学の論文
• 1975-03-31

著者

• Arai Shigeru

  The Nara Prefectural Agricultural Experiment Station

• Osaki Takeshi

  Laboratory Of Plant Pathology College Of Agriculture

関連論文

• The Identification and the Classification of Tulip Breaking Virus and Cucumber Mosaic Virus found Infecting Tulip and Lily Plants
• Transmission Characteristics and Cytopathology of a White fly-Transmitted Virus Isolated from Sweet Potato Leaf Curl Disease
• Studies on the Viruses of Solanaceous Vegetables in Japan. II. A Strain of Tobacco Mosaic Virus Isolated from Pepper Plants
• Biological and Physical Properties of Tobacco Mosaic Virus Mutant Induced by Nitrous Acid
• Necrotic Ring Disease of Japanese Iris (Iris kaempferi Sieb.), A New Disease Caused by Japanese Iris Necrotic Ring Virus.

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