On the Golgi Apparatus of the Cultivated Cells before and after Impregnation with Silver
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概要
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Cells cultivated from the chick heart, kidney, liver and lung were observed in the living condition under the phase contrast microscope to study the structure of the Golgi region and the vacuoles contained in it, and they were then treated by Da Fano's method or Elftman's silver direct impregnation method to demonstrate the classical Golgi apparatus. Inquiries were made into the factors that affected the appearance of the Golgi apparatus and the topographical relation between the vacuoles in the Golgi region of the living cells and the argentophile material demonstrated with the impregnation methods. The argentophile material is usually seen as a clump of threads in the form of a network in the fibroblast, and metamorphoses to be filamentous in appearance in the elongated cell, ring-shaped with an argentophobe center in the well-spread cell and comes to be fragmented in the gigantic cell. The fragmentation of the Golgi apparatus is also found to occur in the fibroblast accumulating a great amount of fat and in the epithelial cell. These results may be thought to indicate that the morphology of the Golgi apparatus depends on the fat formation and the origin, shape and size of the cell. The argentophile material is found usually at the same situation where the Golgi area is located in the living cell, but in some cases the silver deposition is not necessarily found at the same sites where the vacuoles are situated: in some elongated cells, it extends far beyond the sites of the vacuoles. This topographical disagreement between the vacuoles and the argentophile material leads to the conclusion that the vacuoles in the Golgi region observable in the living cells are not necessarily the same with those of the Golgi complex which have been described by the electron microscopists. The present author wishes to express hearty thanks to Prof. Shunzo TAKAGI for his continuous advice and encouragement throughout this work.
- 大阪府立大学の論文
- 1965-01-15