フナおよびオイカワの赤血球を用いた小核試験の河川の汚染モニタリングへの効果的適用

概要

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To evaluate the micronucleus test using fish peripheral erythrocytes to detect genotoxic pollutants in the water environment, we examined the frequencies of micronucleated erythrocytes in the observation of 10,000 erythrocytes per individual in Carassius species and Zacco platypus, captured in upstream, midstream and downstream of Tomio River, which runs through the residential area of Nara. The frequencies were significantly higher in all sampling stations than those in goldfish bred in clean water under the laboratory conditions. But the frequencies of micronucleated erythrocytes in the observations of 3,000 erythrocytes per individual in both species were significantly higher in only a sampling station. These results indicated that the observations of 10,000 erythrocytes per individual were effective to detect genotoxic pollutants more than those of 3,000 erythrocytes per individual. The frequencies of micronucleated immature erythrocytes showed no significant increase in all sampling stations from the control levels.
大阪信愛女学院短期大学の論文
2003-06-25