単一感染動物による歯垢形成に関する実験的研究 : 歯垢量の評価法ならびに比較について

概要

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The present study was carried out to establish the method for evaluating the plaque on the tooth surfaces of experimental animals, and to campare the degree of stained areas with disclosing agents between conventional and mono-infected gnotobiotic mice. ICR: JCL germfree mice as control and gnotobiotic mice by a plaque forming Str. mutans strain PK1 were used. The mono-infected mice were divided into two experimental groups, one was bred with the 10% sucrose added water, another with nonsucrose water, and both were evaluated 1 week after inoculation. The results obtained were follows; 1) The plaque could be stained by tincture of iodine more remarkably than other disclosing agents, and this suited our purpose. 2) The staining index was statistically the smallest in germfree mice among the four groups. 3) The nature of staining in germfree mice was different from the other groups, and the stained materials seemed to be food debris or substances derived from saliva. 4) In the mono-infected mice, sucrose-added group had a tendency to show the larger staining index than non-sucrose group.
特定非営利活動法人日本歯周病学会の論文
1978-03-28