歯垢のmatrixタンパクの抽出について

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In the present study, the amino acid analysis of whole plaque and protein matrix from 2, 4 and 7 day's old plaque was performed and protein matrix was extracted using three kinds of solvents. (trypsin solvent, distilled water and 0.1N NaOH) For comparison, the amino acid compositions of salivary components and several pure cultured bacteria (St. mutans, St. sanguis, Neisseria, Nocardia, Veillonella and A. viscosus) were also analyzed. Isoelectric focusing of protein matrix and whole saliva was performed using two kinds of solvents. (distilled water and 0.1N NaOH) Consequently, the extraction of matrix protein showed that protein content was different in each solvent and each time. The analysis of amino acid composition of matrix protein showed that glutamic acid content was greater in order of that from distilled water, trypsin solvent and 0.1N NaOH extraction. Arginine from trypsin solvent and distilled water extractions decreased as time went by, but that from 0.1N NaOH extraction did not change. Especially arginine content from trypsin solvent extraction decreased remarkably. Several bands of acid protein, neutral protein and alkaline protein were obtained from whole saliva, but several bands of only acid protein (pH4-6) were obtained from plaque matrix extracted by 0.1N NaOH and distilled water in isoelectric focusing. The amino acid compositions of 2, 4 and 7 day's old plaque and plaque matrix were similar each other. The protein bands of 2, 4 and 7 day's old plaque matrix obtained in isoelectric focusing were similar each other too. The amino acid compositions of whole plaque, cultured bacteria, plaque matrix and salivary mucin were found to possess the common characteristics which glutamic acid, asparaginic acid, alanine and glycine were rich.
特定非営利活動法人日本歯周病学会の論文
1978-03-28

歯垢のmatrixタンパクの抽出について

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