Identification and Characterization of Yeasts with Tolerance to High Acidity and Resistance to Aluminum Isolated from Tea Soils (Soil Biology)

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From acidic tea soils in Kagoshima Prefecture in Japan, 38 strains of acid-tolerant microorganisms were isolated and some soil properties were determined. Different Al concentrations were applied to YG media to estimate the Al resistance. Selected microbial strains were able to grow actively in liquid media in the presence of 100 mM Al and to survive even in 300 mM Al at pH 3.0. The base sequences of 28S rDNA-D1/D2 were determined and sequence data were searched using the Basic Local Alignment Search Tool (BLAST) system. The results of sequencing revealed that the isolates belonged to two different species, Cryptococcus sp. and Candida palmioleophila. Subsequently, Neighbor-Joining molecular phylogenetic trees were constructed. When the yeasts were cultured at various Al concentrations, the growth was inhibited at a concentration of 200 mM. Pre-culture of these strains with 0-30 mM Al did not promote the growth response induced by Al. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess the elimination of Al from YG media. The amount of Al remaining in the culture media decreased considerably after culture. Due to a capacity for resistance to significant Al concentrations as well as high Al elimination, these acid-tolerant and Al-resistant yeasts may offer potential applications for the bio- and phyto-remediation of acid soils with a high level of Al.
社団法人日本土壌肥料学会の論文