Contribution of Growth Factors to Heat Production by Cultured Rat Fibroblasts

スポンサーリンク

概要

• 論文の詳細を見る

• The heat released from rat 3Y1 fibroblasts was measured by the stopped-flow method using a flow microcalorimeter. The heat output from a monolayer culture is defined as the maximum heat output from the cell suspension, after dispersion of the monolayer. The heat output per cell was not significantly changed when exponentially proliferating cells ceased dividing, with a G1 DNA content, at confluent cell density. When the growth-arrested (resting) cells were exposed to fresh medium containing various combinations of growth factors, heat output increased in parallel with the fraction of the cells that entered S phase of the cell cycle. The increase in heat output was correlated with the increase in cell volume. We infer from these findings that the resumption of cell proliferation stimulated by growth factors involves an increase in cell volume and a concomitant increase in heat production, but that entry into the resting state does not involve abrupt decreases in cell volume and heat production.

• 東海大学の論文

著者

• OKUDA Atsuyuki

  Department of Virology, Medical Institute of Bioregulation, Kyushu University

• Okuda Atsuyuki

  Department Of Virology Medical Institute Of Bioregulation Kyushu University

• KINURA Genki

  Department of Virology, Medical Institute of Bioregulation, Kyushu University

関連論文

• Induction of DNA Synthesis by Fibroblast Growth Factor in Temperature-Sensitive Cell-Cycle Mutants of Rat 3Y1 Fibroblasts Arrested at Restrictive Temperature
• Reversion of Temperature-Sensitive Mutation by Inhibition of Proteasome-Mediated Degradation of Mutated D123 Protein
• Extensive Degradation of Mutant-Type D123 Protein is Responsible for Temperature-Sensitive Proliferation Inhibition in 3Y1tsD123 Cells
• Contribution of Growth Factors to Heat Production by Cultured Rat Fibroblasts

スポンサーリンク