Bordetella pertussis Adenylate Cyclase : the Gene and the Protein(II. Molecular Biological and Genetic Analysis of Bordetella pertussis)(Current Studies on Future Vaccines)

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概要

• 論文の詳細を見る

• Using the adenylate cyclase-calmodulin interaction as a tool, the B. pertussis cya gene was cloned in a cya defective E. coli strain harbouring a plasmid which expressed high levels of calmodulin. The determination of the nucleotide sequence of the gene showed that adenylate cyclase is synthesized as a large precursor of 1706 amino acids. The calmodulin-stimulated catalytic activity resides in the amino-terminal 400 amino acids whereas the 1300 amino acid carboxy-terminal part of the precursor is endowed with haemolytic activity. The catalytically active 43 kDa form of adenylate cyclase is organized in two domains: the N-terminal domain of 25 kDa harbors the catalytic site, and the 18 kDa C-terminal domain carries the main calmodulin-binding site. Immunological relatedness established between B. pertussis, B. anthracis and rat brain adenylate cyclases suggests a common evolutionary origin of a central domain of these calmodulin-stimulated enzymes. The secretion of the adenylate cyclase-haemolysin bifunctional protein (cyclolysin) requires the expression of three additional genes, contiguous to the cya gene. These four genes appear to form a single operon. The mechanism of secretion of the bifunctional protein should be similar to that described for E. coli alpha-haemolysin.

• 東海大学の論文
• 1988-09-22

著者

• Ullmann Agnes

  Unite De Biochimie Des Regulations Cellulaires Department De Biochimie Et Genetique Moleculaire

• Ladant Daniel

  Unite De Biochimie Des Regulations Cellulaires Department De Biochimie Et Genetique Moleculaire

• GLASER Philippe

  Unite de Regulation de l'Expression Genetique, Department de Biochimie et Genetique Moleculaire

• DANCHIN Antoine

  Unite de Regulation de l'Expression Genetique, Department de Biochimie et Genetique Moleculaire

• BARZU Octavian

  Unite de Biochimie des Regulations Cellulaires, Department de Biochimie et Genetique Moleculaire

• Barzu Octavian

  Unite De Biochimie Des Regulations Cellulaires Department De Biochimie Et Genetique Moleculaire

• Danchin Antoine

  Unite De Regulation De L'expression Genetique Department De Biochimie Et Genetique Moleculaire

• Glaser Philippe

  Unite De Regulation De L'expression Genetique Department De Biochimie Et Genetique Moleculaire

関連論文

• Bordetella pertussis Adenylate Cyclase : the Gene and the Protein(II. Molecular Biological and Genetic Analysis of Bordetella pertussis)(Current Studies on Future Vaccines)

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