Perspectives for Achieving Improved Information by the Observation of Thin Sections in the Electron Microscope(STRUCTURE OF MEMBRANE PROTEINS)

スポンサーリンク

概要

• 論文の詳細を見る

• Obtaining biologically significant fine detailed information from sections is limited firstly by the unknown distribution of heavy metal stain and secondly by conformational changes induced by dehydration. Only afterwards we have to be concerned with beam induced alterations. By Z-imaging in a STEM, completely unstained material can now become imaged sharply with high contrast. By this the first limitation is eliminated and the second can become explored. For this purpose new resins designed for low temperature embedding might become important. First biological results are presented which illustrate the potential of these techniques: The transmembrane protein of the sepatate junction has been revealed as such and shown that only the hydrophilic part of the protein is stained by uranyl acetate. This type of staining therefore does not allow the detection of any transmembrane proteins in sections.

• 東海大学の論文
• 1982-06-14

著者

• Hobot Jan

  Department Of Microbiology Biozentrum Of The University Of Basel

• CARLEMALM Eric

  Department of Microbiology, Biozentrum of the University of Basel

• ARMBRUSTER Barbara

  Department of Microbiology, Biozentrum of the University of Basel

• CHIOVETTI Robert

  Department of Microbiology, Biozentrum of the University of Basel

• GARAVITO R.

  Department of Microbiology, Biozentrum of the University of Basel

• VILLIGER Werner

  Department of Microbiology, Biozentrum of the University of Basel

• KELLENBERGER Edward

  Department of Microbiology, Biozentrum of the University of Basel

• Garavito R.

  Department Of Microbiology Biozentrum Of The University Of Basel

• Carlemalm Eric

  Department Of Microbiology Biozentrum Of The University Of Basel

• Armbruster Barbara

  Department Of Microbiology Biozentrum Of The University Of Basel

• Villiger Werner

  Department Of Microbiology Biozentrum Of The University Of Basel

• Chiovetti Robert

  Department Of Microbiology Biozentrum Of The University Of Basel

• Kellenberger Edward

  Department Of Microbiology Biozentrum Of The University Of Basel

• GARAVITO R.

  Department of Biochemistry and Molecular Biology, Michigan State University

関連論文

• Perspectives for Achieving Improved Information by the Observation of Thin Sections in the Electron Microscope(STRUCTURE OF MEMBRANE PROTEINS)
• Biochemical characterization of human dynamin-like protein 1

スポンサーリンク