Image Analysis of Electron Micrographs of ATPase (Coupling Factor TF_1) from Thermophilic Bacteria and Luminal Epithelium of Mouse Urinary Bladder(STRUCTURE OF MEMBRANE PROTEINS)

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概要

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• Electron micrographs of two dimensional array of H^+ -ATPase (Coupling Factor TF_1 from the thermophilic bacteria) and luminal epithelial protein of urinary bladder were obtained using negative staining method and freeze-fracture method, respectively. Images which showed good optical diffraction pattern were digitized by a computer-linked flat-bed two dimensional microdensitometer and processed digitally. The results of translational computer noise filtering showed that the outline of TF_1 molecules looks like a hexagon or an asterisk in the presence of sodium azide which is a specific inhibitor of TF_1 or AMPPNP which is an unsplitable analogue of ATP, respectively. The luminal epithelial protein also looks like a hexagon. Rotational harmonic analysis was carried out to examine the rotational symmetry of the filtered images of TF1. It was found that 2-fold or 6-fold symmetry is dominant in the presence of sodium azide or AMPPNP, respectively.

• 1982-06-14

著者

• Kagawa Yasuo

  Department Of Biochemistry Faculty Of Medicine Jichi Medical School

• TOMIOKA Akihiro

  Department of Applied Physics, Faculty of Engineering, University of Tokyo

• Wakabayashi Takeyuki

  Department of Biosciences, School of Science and Engineering, Teikyo University

• Usukura Jiro

  Department of Anatomy, Nagoya University School of Medicine

• Yoshida Masasuke

  Department of Biotechnology, Kyoto Sangyo Univ.

• Usukura Jiro

  Department Of Anatomy Faculty Of Medicine University Of Tokyo

• Usukura Jiro

  Department Of Anatomy And Cell Biology Nagoya University

• TOKUNAGA Makio

  Department of Physics, Faculty of Science, University of Tokyo

• YAMADA Eichi

  Department of Anatomy, Faculty of Medicine, University of Tokyo

• Yamada Eichi

  Department Of Anatomy Faculty Of Medicine Kyushu University

• Tokunaga Makio

  Department Of Physics Faculty Of Science University Of Tokyo

• Yoshida Masasuke

  Department Of Biochemistry Faculty Of Medicine Jichi Medical School

• Tomioka Akihiro

  Department Of Physics Faculty Of Science University Of Tokyo

• Tomioka Akihiro

  Department Of Applied Physics Faculty Of Engineering University Of Tokyo

• Wakabayashi Takeyuki

  Department Of Physics Faculty Of Science University Of Tokyo

• Wakabayashi Takeyuki

  Departemnt Of Physics Faculty Of Science The University Of Tokyo

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