Oxalate Enhances Protein Synthesis in Cell-Free Synthesis System Utilizing 3-Phosphoglycerate as Energy Source(METHODS)

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概要

• 論文の詳細を見る

• Herein, we report our results showing that the productivity of cell-free protein synthesis can be enhanced through the regulation of the in vitro metabolism of an energy source. In a reaction mixture utilizing 3-phosphoglycerate (3PG) as an energy source, the supply of ATP was significantly enhanced when the reaction mixture was supplied with sodium oxalate, a potent inhibitor of phosphoenolpyruvate synthetase (PPS). The productivity of protein synthesis was also increased by approximately 70% upon the addition of oxalate. It was presumed that this enhancement in ATP supply resulted from the prevention of the pyruvate→PEP reaction, which causes nonproductive ATP consumption. For the initial presence of 2.1 mM sodium oxalate, approximately 720 μg/ml chloramphenicol acetyltransferase (CAT) was produced after 3 h of incubation at 37℃.

• 公益社団法人日本生物工学会の論文
• 2006-02-25

著者

• Choi Cha-yong

  School Of Chemical And Biological Engineering Seoul National University

• Kim Tae-wan

  School Of Chemical And Biological Engineering Seoul National University

• Park Chang-gil

  Department Of Fine Chemical Engineering And Chemistry Chungnam National University

• Kim Dong-myung

  Department Of Fine Chemical Engineering And Chemistry Chungnam National University

• Kuem Jung-Won

  School of Chemical and Biological Engineering, Seoul National University

• Kuem Jung-won

  School Of Chemical And Biological Engineering Seoul National University

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