Improved L-Threonine Production of Escherichia coli Mutant by Optimization of Culture Conditions(BREWING AND FOOD TECHNOLOGY)
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概要
- 論文の詳細を見る
L-Threonine production was investigated in a minimal salt medium using L-threonine-overproducing Escherichia coli MT201, derived from E. coli K-12. It was observed that dry cell weight reached 12.5 g/l with 15.9 g/l L-threonine. To increase dry cell weight and L-threonine production, the fermentation process was optimized. When biotin was added as growth factor, L-threonine production reached 52.0 g/l from 15.9 g/l without biotin. Dry cell weight and L-threonine production were further increased by continuous feeding of the feed media with an optimized L-methionine concentration (5.0 g/l). However, high-cell-density culture caused oxygen-limited condition, which resulted in the accumulation of organic acids. To overcome this problem, oxygen-enriched air was supplied to the fermentor with the minimal salt medium. Under these optimal conditions, we achieved an L-threonine production of 80.2 g/l in the minimal salt medium.
- 社団法人日本生物工学会の論文
- 2006-02-25
著者
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Park Jin-ho
Division Of Food And Biotechnology Kyungnam University
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Ahn Jung-oh
Korea Research Institute Of Bioscience And Biotechnology
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HWANG Yong-Il
Division of Food Science and Biotechnology, Kyungnam University
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Lee Hong-weon
Korea Research Institute Of Bioscience And Biotechnology
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Jung Joon-ki
Korea Research Institute Of Bioscience And Biotechnology
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Hwang Yong-il
Division Of Food And Biotechnology Kyungnam University
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Lee Man-Hyo
Division of Food and Biotechnology, Kyungnam University
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Lee Man-hyo
Division Of Food And Biotechnology Kyungnam University
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Hwang Yong-Il
Division of Food and Biotechnology, Kyungnam University
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