リポ多糖体刺激ラット組織におけるToll-Like Receptor 4の発現状況の解明
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In this study, an expression of Toll-Like Receptor 4 (TLR-4) in the rat gingiva after lipopolysaccharide(LPS) stimulation was examined by means of immunohistochemistry. Wistar rats, 6 weeks of age, were used. A five μl of saline containing 25μg of LPS from E. coli was injected into the palatal gingiva at the first molar of LPS-sensitized or non-sensitized rats. After perfusion fixation with PLP fixative, the gingiva was excised. Serial frozen sections were cut and stained immunohistochemically using polyclonal antibody against TLR4 and monoclonal antibody against monocyte/macrophage (EDl). The gingival epithelium and capillary endothelial cells consistently expressed TLR4 not only in the LPS-injected groups but also in the saline-injected group and the non-treated normal control. The injection of LPS to normal (non-sensitizing) rat gingiva brought about an infiltration of significant amount of neutrophils and mononuclear cells in the connective tissue after 12 to 24 and 24 to 36 hours, respectively. Most of the neutrophils and about a half of the mononuclear cells expressed TLR4. Most of the mononuclear cells showed positive immunostaining for EDl, some of which were dendritic or stellete in shape and exhibited nuclear indentation. In the LPS-sensitized rats, re-challenge of LPS resulted in higher infiltration of both neutrophils and mononuclear cells than in the non-sensitized rats and those infiltrates were observed for longer period. A ratio of TLR4-positive mononuclear cells to the whole mononuclear cells appeared to increase. The present results suggested that the gingival epithelium, capillary endothelial cells and infiltrating neutrophils constitutively expressed TLR4 and mononuclear cells expressed it in a stimulation-dependent manner. Both the gingival epithelium and capillary endothelial cells may play a part in the innate immune response in the rat gingiva.
- 2005-06-30
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関連論文
- リポ多糖体刺激ラット組織におけるToll-Like Receptor 4の発現状況の解明
- 4)リポ多糖体刺激ラット組織におけるToll-Like Receptor4の発現状況の解明(第38回 奥羽大学歯学会例会講演抄録)