STUDIES ON THE LEAF NODULES VII : PROPERTIES IN PURE CULTURE OF THE LEAF NODULE BACTERIUM ISOLATED FROM THE HOST-PLANT, ARDISIA CRISPA (THUNB.) A. DC. AND A. PUNCTATA LINDL
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In Japan Hanada found a new leaf nodule bacterium, Xanthomonas hortoricum Hanada, which was never found in any other countries by the investigators and in Japan never from other plants than the horticultural variety "Ohbatarayo" of Ardisia hortorum. But in Japan strains of true Bacterium foliicola Miehe symbioses not only with A. crispa as found by Miehe it tropical regions, but also with wild A. punctata. As the habitual companion of B. foliicola, a species different from thai of Miehe was found in Japan, but that in Japan coincides with those found in Holland and Germany by de Jongh and Glaubitz from the garden plants perhaps brought from tropics. On the affined A. japonica which grew mingled with the above mentioned leaf nodulous species and never bear leaf nodules, no nodulous bacteria was found from any part of the plant. This species rejects symbiotic bacteria got from other nodulous plants. Miehe and de Jongh isolated B. foliicola from germinating seeds of A. crispa and from the same species Glaubitz also isolated K and V strains the fomer from germinating seeds and the latter from growing points of seedings. But he identified the two strains as Mliehe's B. foliicola in spite of the existence of some morphological and physiological differences between them. In Japan the author isolated C_2 strain on A. crispa and P_3 on A. punctata from their germinating seeds respectively. They were all motile reds. No remarkable differences were found on their morphological characteristics. On one month old pea-extract slant-culture, colony surface of C_2 strain became more or less rough and dull color but that of P_3 smooth and glossy. No striking differences were found on gelatin and bouillon stabculture. On the plate-culture of potato ager, P_3 grew better and became different shape than the others. In liquid-culture of pea-extract and bouillon C_2 was superior in membrane and sediment formation, and PH value of C_2 was higher than P_3. Color and viscosity in pea-extract suspension P_3 was more browish and less slimy