マウス白血病細胞への薬剤耐性遺伝子の移入と分化誘導

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概要

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• The gene transfer into, various target cells is an important technique in genetic engineering, for such as biomass production or gene therapy because of the critical role it plays in new protein synthesis and intermediary metabolism. We demonstrate here, using a laser microinjector, the introduction of a drug-resistance gene into cultured-murine cells (TSA8). The cells were transformed to Geneticin (G418) resistance by introduction of the neomycin-resistanc gene. The transformed-murine erythroleukemia cells could be in- duced to differentiate by dimethyl sulfoxide (DMSO). Erythroid differrentiation by vitamin D_3 derivatives was examined in the cells. After the cells were cultured for 5-days with 1.0% DMSO, as much as 60% of .the cells became benzydine-positive. This differentiation was markedly inhibited by addition of the active form of vitamin D_3, 1α, 25-dihydroxyvitamin D_3 (1α, 25 (OH)_2D_3). The active vitamin D_3 was the most potent in inhibition for the DMSO-induced erythroid differentiation. These results are the same results that the vitamin D_3 derivatives and DMSO are involved in erythroid differentiation of normal murine cells, as published elsewhere. These results suggest that the transduction of neomycin-resistance genes is totally ineffective in cell growth and erythroid differentiation for murine cells. These experiments also provide a posibility.or model for future various gene replacement therapy in which functional genes can be introduced into various target cells using the laser microinjector.

• 宮崎女子短期大学の論文

著者

• 山越 智

  Institute Of Applied Microbiology University Of Tokyo

• 大石 道夫

  Institute Of Applied Microbiology University Of Tokyo

• 日高 英幸

  Miyazaki Women's Junior College

関連論文

• マウス白血病細胞への薬剤耐性遺伝子の移入と分化誘導

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