ラット頚動脈バルーン傷害モデルにおける新生内膜でのCoxsackie Virus and Adenovirus Receptor(CAR)の発現とその意義

概要

論文の詳細を見る
Backgrounds: Coxsackievirus and adenovirus receptor (CAR) belongs to immunoglobulin superfamily and functions as a high-affinity receptor for the two viruses. CAR expression is abundant in the brain and heart during embryo and declines to a trace level after birth. Though CAR is re-expressed in the injured cardiac muscle, its biological function and regulatory mechanism is to be known. Methods and Animals: To extend our previous study, we investigated the involvement of CAR in the balloon-injured artery in rats. CAR protein and the mRNA were studied on days 3 to 21 after balloon injury. CAR expression was also studied in primarily cultured vascular smooth muscle cells (SMCs) obtained from rat aorta. Results: CAR was observed in endothelial cells (ECs) but not in the subendothelial cells before injury. On day 5 after injury, CAR was expressed strongly in the innermost layer of medial SMCs. On day 7, neointimal SMCs appeared with a strong expression of CAR simultaneously with proliferating cell nuclear antigen (PCNA), while CAR in medial SMCs onserved on day 5 disappeared. On day 14, CAR disappeared from the neointima and was expressed in regenerated ECs. CAR mRNA increased to a peak level on day 7 (p = 0.005) and then decreased gradually there-after. CAR protein expression was also confirmed in proliferating SMCs in culture. Conclusion: The intimal hyperplasia was associated with CAR expression in the rat carotid artery.
新潟大学の論文
2003-09-10