<原著>培養グリオーマ細胞に対する温熱と抗癌剤の併用効果に関する研究
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概要
- 論文の詳細を見る
Antineoplastic agents have been reported to have little effect against malignant brain tumors. In the present study, the synergistic cytotoxicity of combination with hyperthermia and antineoplastic agents was investigated using glioma cell lines. The glioma cell lines used in this study include rat glioma cell lines C6,9L and human glioma cell line T98G. These cell lines were cultured with antineoplastic agents of ADR, CDDP, and MCNU with various concentrations and temperature. The cell survival of each cell line exposed to drugs of different concentration combined with various temperature stimulation was detected with WST-1 assay. The cytotoxic effect was related to the temperatures and duration of exposure to the temperatures and duration of exposure to the temperature when the hyperthermia was done alone. The cytotoxicity increased according to the drug concerntration when the drug were used alone. The cytotoxicity increased significantly if the cell line were cultured at 43℃ for 1 hour combined with 0.5μg/ml of ADR, CDDP or 2.5μg/ml of MCNU (p<0.01) compared with that of hyperthermia group or antineoplastic agents group assessed by combination index (CI) method. Combined therapy with hyperthermia and antineoplastic agents has synergistic cytotoxic effect on cultured glioma cell lines. WST-1 assay is more useful for assessing cell survival than colony assay or MTT assay.
- 新潟大学の論文
- 2002-06-10