CCD生体顕微鏡を用いた絞扼による虚血再潅流時の腫瘍組織の微小循環の観察と抗腫瘍効果の検討

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The purpose of this study was to examine the antitumor effect of strangulation on tumor tissue, AH130, implanted in the murine mesentery, near the ileocecal portion 10 days previously. A recovery rate of blood flow was observed after reperfusion with a charge-coupled device microscope. Five days after reperfusion, the tumor volume was measured. On the death or sacrifice of the animals, the tumor necrotic area and the morphological changes of the cecal mucosa were examined. In each strangulated group, 60 and 90-minutes strangulation, a recovery rate of blood flow was lower in tumor tissue than in the normal tissue around tumor tissue. The tumor volume decreased and the tumor necrotic area increased more in the strangulated group than in the nonstrangulated group. Among all strangulated groups, the longer the strangulated time was, the more changes were observed. The 30-day survival rate after tumor implantation was better in animals with 60 and 90-minutes strangulation than in those with 15 and 30-minutes strangulation and without. These data suggested that the tolerance of tumor cells and tumor endothelial cells to strangulation, which might cause ischemia-reperfusion, is less than that of normal cells and normal endothelial cells.(CCD)
岐阜大学の論文
2003-03-31