<Original article>Effects of EGF on Cell Migration, and the Association of FAK and Paxillin to the Cytoskeleton

スポンサーリンク

概要

• 論文の詳細を見る

• We investigated the effects of epidermal growth factor (EGF) on cell migration, and the association of focal adhesion kinase (FAK) and paxillin to cytoskeleton. A cell line of squamous cell carcinoma (SCC) of the uterine cervix, named Caski, was used. We examined the effects of different concentrations of EGF (0, 1, 10 and 100 ng/ml) on cell migration. Wound closure assay showed that cell migration within 4 h had a biphasic dose-response curve on the concentration of EGF, and that cell migration was stimulated at 1 and 10 ng/ml, with a maximum at 10 ng/ml, and suppressed at 100 ng/ml among the concentrations examined. Immunoblot and immunoprecipitation assays of whole cell lysates obtained at 20 min after the addition of EGF demonstrated that the phosphotyrosine level of FAK and paxillin had also a biphasic response curve dependent on EGF concentration, and a maximum level was observed at 10 ng/ml among the concentrations employed. To examine the amount of cytoskeleton-associated fractions of these proteins, they were extracted after a rinse in non-ionic detergent, and analyzed by immunoblot assay. At 30 min the amount of FAK and paxillin did not differ among the concentrations examined. At 90 min, however, the amount of both proteins revealed a reverse biphasic dose-response curve, with a maximal reduction at 10 ng/ml among the concentrations examined. Integrins and actinin were not changed at each concentration of EGF examined. Immunofluorescence microscopy showed that at 30 min the expression of actin stress fibers (ASF) did not differ among cells treated with each concentration of EGF. At 90 min the ratio of cells that expressed a small number of ASF and focal adhesions (FA) was significantly increased at 10 ng/ml. Although the ratio of cells that expressed large numbers of these structures was not significantly altered, ASF were gathered at the center of cells and tangled at 100 ng/ml. These data suggest that the modulation of cytoskeletal organization by EGF plays a role in the EGF-induced stimulation of cell migration.

• 新潟大学の論文

著者

• ITO Masaaki

  Division of Colorectal and Pelvic Surgery, Department of Surgical Oncology, National Cancer Center H

• Ito M

  Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica

• Ito K

  Niigata Univ. School Of Medicine Niigata Jpn

• CHIBA Hitomi

  Division of Dermatology, Department of Cellular Function, Niigata University Graduate School of Medi

• KAZAMA Takashi

  Division of Dermatology, Department of Cellular Function, Niigata University Graduate School of Medi

• YAMAMOTO Yoko

  Division of Dermatology, Department of Cellular Function, Niigata University Graduate School of Medi

• SUYAMA Takayuki

  Division of Dermatology, Department of Cellular Function, Niigata University Graduate School of Medi

• Chiba Hitomi

  Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica

• Kazama Takashi

  Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica

• Suyama Takayuki

  Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica

• Ito Masaaki

  Division Of Colorectal And Pelvic Surgery Department Of Surgical Oncology National Cancer Center Hos

• Yamamoto Yoko

  Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica

関連論文

• Diagnosis supporting algorithm for lymph node metastases from colorectal carcinoma on ^F-FDG PET/CT
• Improvement of the diagnostic accuracy of lymph node metastases of colorectal cancer in ^F-FDG-PET/CT by optimizing the iteration number for the image reconstruction
• Venous Thromboembolism : Deep Vein Thrombosis With Pulmonary Embolism, Deep Vein Thrombosis Alone, and Pulmonary Embolism Alone(Hypertension/Pulmonary Circulation)
• Bladder-Sparing Extended Resection of Locally Advanced Rectal Cancer Involving the Prostate and Seminal Vesicles
• NK cells contribute to the skin graft rejection promoted by CD4^+ T cells activated through the indirect allorecognition pathway
• Up-regulation of cell surface Toll-like receptor 4-MD2 expression on dendritic epidermal T cells after the emigration from epidermis during cutaneous inflammation
• Predictors of Successful Salvage Surgery for Local Pelvic Recurrence of Rectosigmoid Colon and Rectal Cancers
• Effects of EGF on Cell Migration, and the Association of FAK and Paxillin to the Cytoskeleton
• Characterization of factor XIIIa+ dendritic cells in dermatofibroma : Immunohistochemical, electron and immunoelectron microscopical observations
• A Case of Trichoepithelioma Papulosum Multiplex with Sweat Duct Differentiation : An Immunohistochemical and Electron Microscope Study
• Silent gastric perforation in a pancreatic cancer patient treated with neurolytic celiac plexus block
• Comparisons of the Laccase Gene among Serotypes and Melanin-Deficient Variants of Cryptococcus neoformans
• Adult Henoch-Schonlein Purpura with Severe Abdominal Pain Treated with Dapsone and Factor XIII Concentrate
• An Ultrastructural Study of Cutaneous Adnexal Carcinoma with CombinedFolliculosebaceous and Apocrine Differentiation
• Ultrastructural Study of Acquired Pili Torti-like Hair Defects Accompanying Pseudopelade
• Prognosis of 100 Japanese patients with mycosis fungoides and Sezary syndrome
• Association Between Incisional Surgical Site Infection and the Type of Skin Closure After Stoma Closure
• Surgical management of small bowel metastases from primary carcinoma of the lung
• Postoperative chylous ascites after colorectal cancer surgery

もっと見る 閉じる

スポンサーリンク