The Enterohemorrhagic Escherichia coli (EHEC) Hemolysin Genes of aShiga Toxin (Stx1)- and Stx2-Producing, Serotype O128 Escherichia coliStrain with a Greatest Hemolytic Activity

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Most strains of enterohemorrhagic Escherichia coli (EHEC) belonging to serotypes O157,O26,O111,and O128,isolated from humans in Japan also produced EHEC-hemolysin (EHEC-hly), the hemolytic activity of whom was detected on blood agar containing washed but not unwashed sheep erythrocytes. Of those, Shiga toxin 1 (Stx1)- and Stx2-producing strains belonging to serotypes O128 : H2 and O128 : H12 produced EHEC-hly in much greater quantities than did the other strains. The two strains carried the EHEC-hly genes on the 120-kbp plasmid. The entire EHEC-hly region (carrying genes C, A, B, and D) of the serotype O128 : H12 strain was cloned into pACYC184 and sequenced. The EHEC O128-hly genes showed a close similarity to the the EHEC O157-hly genes of more than 98.3% at the nucleotide level (more than 97.7% at the amino acid level). However, the EHEC O128-hlyB (the ATP-binding transmembrane protein acting as a transporter for hemolysin [hlyA]) lacked one amino terminal methionine, in contrast to the repeated amino terminal methionines in the EHEC O157-hlyB. The O128- and O157-hly As shared similar RTX repeats and acylation sites.
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