The Efficiency of Adoptive Immunotherapy for Ehrlich Tumors in Miceand Fate of the Transferred Spleen Cells in the Recipient

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概要

• 論文の詳細を見る

• It has been shown that adoptive immune transfer in mice for Ehrlich ascites tumors (EAT) is successful in a genetically defined combination such as B6 as donor and B6-nu/nu as recipient. Among allogeneic mouse strains, the adoptive immune transfer was rarely successful with a few exceptions. To observe the mechanism, the fate of intraperitoneally transferred donor spleen cells in the recipients was monitored by staining the cells with fluorescent PKH-26 dye, and using some typical donor and recipient combinations. The results are as follows. Spleen cells from B6-Ly-2^a, 3^a mice were very effective in suppressing subcutaneous EAT outgrowth in B6-nu/nu mice but not in DBA/1 or in ddY-drm. Spleen cells of B6-Ly-2^a, 3^a mice were alive and infiltrated everywhere in B6-nu/nu mice. Some of the effector cells both reached and made contact with EAT cells and lethally affected the tumor cells. Accordingly, EAT outgrowth in B6-nu/nu mice was remarkably suppressed and no solid tumor formation was recognized. On the other hand, spleen cells from B6-Ly-2^a, 3^a mice were killed at an early stage by peritoneal macrophages in the recipients in DBA/1 or in ddY-prg mice, and they did no reach the EAT site. As a result, subcutaneous EAT progressed gradually and formed a solid tumor. The spleen cells of B6-Ly-2^a, 3^a mice which were subcutaneously inoculated into the recipients in a mixture form with EAT cells were sufficiently active for suppressing EAT outgrowth both in DBA/1 and ddY-prg mice. From the results, it is concluded that the contact of donor spleen cells with EAT cells in the recipient without any disturbance is highly important in producing a suppressive effect on EAT in vivo. At an early stage in the spleen cell transfer, recipient macrophage activity is the first barrier to the effective donor cells transferred.

• 新潟大学の論文

著者

• Sato Norimitsu

  Institute For Laboratory Animals Niigata University School Of Medicine

• Norimitsu L.

  Institute For Laboratory Animals Niigata University School Of Medicine

• Akiko Kato

  Institute For Laboratory Animals Niigata University School Of Medicine

• KATO Akiko

  Institute for Laboratory Animals, Niigata University School of Medicine

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