キャピラリー電気泳動法による医薬品製剤成分の一斉分析とインキャピラリー酵素反応の応用研究

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In this thesis, simultaneous determination methods for ingredients in pharmaceutical preparations were studied by capillary electrophoresis (CE). A following study involved applications of an in-capillary enzyme reaction to pharmaceutical analyses. The author studied it while considering that the new method will become a standard analytical procedure for pharmaceuticals of the next generation. First, several separation modes of CE were applied for each dosage form. That is, micellar electrokinetic chromatography (MEKC), cyclodextrin-modified MEKC, hydrophobic interaction electrokinetic chromatography, and cyclodextrin-modified microemulsion electrokinetic chromatography were applied to a vitamin-enriched drink, ophthalmic solutions, an ointment, and cold medicine, respectively. Next, the in-capillary enzyme reaction method was applied to pharmaceutical analysis for the first time. The method was used to determine riboflavin phosphate in a vitamin-enriched drink based on its conversion to riboflavin with alkaline phosphatase. It was then also applied to determine the total amount of chondroitin sulfate based on its conversion to desulfated chondro-disaccharide with chondroitinase ABC and chondrosulfatases. Finally, a scheme for the selection of the CE separation mode of every dosage form was proposed. Several applications and in-capillary enzyme reaction techniques presented in this thesis gave encouraging results in pharmaceutical analysis.
2006-03-05