Studies on the Regeneration of Epidermis in Fresh-water Planarian, Dugesia japonica

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1. This study was performed in order to elucidate the origin of regenerated epidermis, as well as the regeneration process, in the Japanese fresh-water planarian, Dugesa japonica ICHIKAWA et KAWAKATSU. For this purpose, regeneration of epidermis after transverse cutting of the animal at the prepharyngeal or postpharyngeal region, and after removal of a small patch of dorsal epidermis, was observed. 2. In thirty minutes after operation, an epithelioid has formed by the extension of the adjacent old epidermis, to cover the wound surface, at about 17 hours after cutting, accumulation of neoblasts begins, resulting in the falling off of the epithelioid. The upper layer of the neoblasts differentiates into the new epidermis within 50 hours after cutting. 3. With the growth of the upper layer, the differentiating cells in the underlying layer become flat, clearly distinguishable from those of the upper layer, at about 40 hours after cutting. These flat cells secrete the basal membrane, which is complete in about 60 hours after cutting. 4. The distinction between the new and the old epidermis was made by difference in the degree of staining with the Giemsa's solution, and by the absence or presence of the basal membrane, which was revealed by haematoxylin staining. 5. From these observations it is concluded that the new epidermis originates from the neoblasts. 6. The growth of the new epidermis is considered to be effected by the migration of additional neoblasts passing through the basal membrane, because, first, few mitotic figure was detected in the new epidermis even in colchicine-treated samples, and, second, neoblasts were after observed passing through the basal membrane. We believe that similar conclution holds also for old epidermis, but this point still requires further confirmation.
大阪教育大学の論文

Studies on the Regeneration of Epidermis in Fresh-water Planarian, Dugesia japonica

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