Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines

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概要

• 論文の詳細を見る

• Cytocidal effects of anti-cancer drugs such as cisplatin, 5-fluorouracil (5-FU), and actinomycin D on various cell lines were investigated using Alamar Blue. Actinomycin D, cisplatin and 5-FU showed significant cytocidal effects at 1μg/ml, 10μg/ml and 50μg/ml respectively, on cultured cells after 24 h incubation at 37℃. The cytocidal effects increased at a low cell density but reduced at a high cell density in various cell lines. The expression level of one of the ribosomal proteins, nbl (S3a), was measured by northern blot analysis and real time-polymerase chain reaction. The relationship between the nbl mRNA level and drug effects was then investigated for 8 rat and 10 human cell lines. An inverse correlation between chemosensitivity and nbl mRNA level was observed in both rat and human cell lines.

• 獨協医科大学の論文
• 2004-03-25

著者

• Nemoto Kazuhiro

  First Department Of Surgery Dokkyo University School Of Medicine

• Nemoto Kazuhiro

  First Department of Surgery, Dokkyo University School of Medicine

関連論文

• Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines

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