Methyl-β-cyclodextrin Treatment Affects the Thermotropic Behaviour of Membranes and Detergent-Resistant Membrane Fractions of Cultured A431 Cells(Molecular and Cell Biology)

概要

論文の詳細を見る
Membranes and detergent-resistant membrane fractions isolated from human epidermoid carcinoma A431 cells after treatment with methyl-β-cyclodextrin, a compound commonly used in pharmaceutical applications and in manipulation of membrane cholesterol content, display thermotropic transitions at about 15℃ and above 37℃, respectively, when analyzed by differential scanning calorimetry. The transitions, absent in untreated cells, were reversible upon cycling through heating and cooling scans, and attributable to lipid components of the membranes, possibly sphingolipids. These results suggest that, after treatment with methyl-β-cyclodextrin, membranes may show thermotropic transitions, an unusual feature for cellular bilayers, which is likely to influence biological functions.
公益社団法人日本薬学会の論文
2005-12-01