Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines
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概要
- 論文の詳細を見る
The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3Gy/min) or at pulsed low dose rate (p-LDR, average 1Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect.
- 2004-10-10
著者
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Cate R.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Ten Cate
Department Of Cardiology And Endodontology Academic Centre For Dentistry Amsterdam (acta)
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Kreder N.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Castro Kreder
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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RODERMOND H.
Department of Radiotherapy, Academic Medical Center, University of Amsterdam
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BREE C.
Department of Radiotherapy, Academic Medical Center, University of Amsterdam
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FRANKEN N.
Department of Radiotherapy, Academic Medical Center, University of Amsterdam
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ZDZIENICKA M.
Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center
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HAVEMAN J.
Department of Radiotherapy, Academic Medical Center, University of Amsterdam
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Bree C.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Rodermond H.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Franken N.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Haveman J.
Department Of Radiotherapy Academic Medical Center University Of Amsterdam
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Zdzienicka M.
Department Of Radiation Genetics And Chemical Mutagenesis Leiden University Medical Center
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