The Combination of Suicide Gene Therapy and Radiation Enhances the Killing of Nasopharyngeal Carcinoma Xenographs

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概要

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• Nasopharyngeal carcinoma (NPC) is very common in Southern China and Southeast Asian countries. To explore a novel and more effective approach to NPC therapy, a combined strategy of suicide genes and radiation was designed in this study. Five suicide gene expression cassettes, yeast CD, yeast CD/UPRT, and yeast CDglyTK gene controlled by CMV, and Egr-1 and a synthetic CMV-enhanced Egr-1 promoter (CE) were constructed in an expression vector p11MS. The expression of suicide genes in NPC CNE-2 cells were detected by RT-PCR and Western blot. The cytotoxicity of suicide gene therapy and radiation were analyzed by MTT assay. An animal study in which yeast CD/UPRT-expressing CNE-2 tumors in nude mice were treated with 5-FC and radiation was also developed. Our results revealed that p11MSCEyCD/UPRT and p11MSCEyCDglyTK are superior over three other constructs in the killing of NPC cells in vitro. We combined suicide gene-expressing tumors, 5-FC treatment, and radiation in vivo and found that the tumors greatly regressed, some disappeared completely in 3 nude mice in the yCD/UPRT group, and a significant difference of tumor volumes was observed between this group and the other four groups (p<0.05). Our results indicated that suicide gene therapy and radiation have a synergic effect on NPC therapy, and the combined strategy of radiogene therapy is of great potential as a substitute for the traditional method, radiation alone, in NPC therapies.

• 日本放射線影響学会の論文
• 2004-06-15

著者

• Tang A

  Central South Univ. Changsha Chn

• Chen Zhuchu

  Xiangya Hospital Of Central South University

• XIA Jiahui

  National Laboratory of Medical Genetics of China, Central South University

• XIA Kun

  National Laboratory of Medical Genetics of China, Central South University

• FENG Yong

  E.N.T Department of Xiangya Hospital, Central South University

• TANG Aifa

  National Laboratory of Medical Genetics of China, Central South University

• TANG Yaoyun

  E.N.T Department of Xiangya Hospital, Central South University

• WU Lingqian

  National Laboratory of Medical Genetics of China, Central South University

• LIANG Desheng

  National Laboratory of Medical Genetics of China, Central South University

• CAI Fang

  National Laboratory of Medical Genetics of China, Central South University

• PAN Qian

  National Laboratory of Medical Genetics of China, Central South University

• LONG Zhigao

  National Laboratory of Medical Genetics of China, Central South University

• DAI Heping

  National Laboratory of Medical Genetics of China, Central South University

• ZHANG Yunyi

  E.N.T Department of Xiangya Hospital, Central South University

• ZHAO Suping

  E.N.T Department of Xiangya Hospital, Central South University

• Tang Aifa

  National Laboratory Of Medical Genetics Of China Central South University

• Cai Fang

  National Laboratory Of Medical Genetics Of China Central South University

• Dai Heping

  National Laboratory Of Medical Genetics Of China Central South University

• Long Zhigao

  National Laboratory Of Medical Genetics Of China Central South University

• Liang Desheng

  National Laboratory Of Medical Genetics Of China Central South University

• Tang Y

  E.n.t Department Of Xiangya Hospital Central South University

• Wu Lingqian

  National Laboratory Of Medical Genetics Of China Central South University

• Zhao Suping

  E.n.t Department Of Xiangya Hospital Central South University

• Zhang Yunyi

  E.n.t Department Of Xiangya Hospital Central South University

• Pan Qian

  Central South Univ. Hunan Chn

関連論文

• The Role of Enhanced Radiosensitivity and Tumor-specific Suicide Gene Vector in Genetherapy of Nasopharyngeal Carcinoma
• The Combination of Suicide Gene Therapy and Radiation Enhances the Killing of Nasopharyngeal Carcinoma Xenographs
• A novel locus for autosomal dominant nonsyndromic hearing loss identified at 5q31.1-32 in a Chinese pedigree

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