膀胱腫瘍の器官培養 : (2)高圧下器官培養法の臨床的応用

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The method of organ culture under hyper-baric oxygen was applied in the present report for the determination of in vitro effects of several anticancer agents onto human bladder carcinomas. Anticancer agents currently being used on clinical cases of bladder carcinoma, such as Mitomycin C, Thio-Tepa and saccharolactone were added to the culture medium at each concentration to be tested. Tissues or transitional cell carcinoma grade 1 to 3 from 21 cases were cultured under 2 atmospheres for 48 hours, temperature at 37℃ and pH at 7.2 to 7.4. Cultured tissues were studied histologically, electronmicroscopically and also studied with autoradiography. The autoradiographs were made by ^3H-thymidine added to the medium at the last 2 hours of culture period. In control cultures, tissues from grade 2 of transitional cell carcinoma were well maintained under 2 atmospheres for 48 hours and autoradiographs revealed that the labeling indices of the superficial zone of the explants were higher than the deeper zone where cancer cells were still synthesizing DNA. In cultures with Mitomycin C 1.0 meg per ml, cancer cells near the surface of explants were markedly degenerated, while the cells in deeper zones partly survived and the labeled cells were seen. In cultures with Thio-Tepa 1.0 meg per ml cancer cells of the entire specimen were degenerated. The DNA synthesizing cells were not recognized so far with ^3H-thymidine autoradiography of Thio-Tepa group. In cultures with saccharolactone 1.0 meg per ml, the degenerated foci were seen in entire specimens but the degree of degeneration was slight. By electronmicroscopy, cancer cells near the basement membrane showed deformities of nuclei and nucleoli with a minimum dosis of Thio-Tepa, while the cytoplasmic organellae were affected with Mito-mycin C.
社団法人日本泌尿器科学会の論文

膀胱腫瘍の器官培養 : (2)高圧下器官培養法の臨床的応用

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