One-step Sandwich Enzyme Immunoassays for Human Matrix Metalloproteinase 13(Collagenase-3)using Monoclonal Antibodies

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概要

• 論文の詳細を見る

• Two one-step sandwich enzyme immunoassay(EIA)systems for human matrix metalloproteinase 13(MMP-13, collagenase 3)have been established with two pairs of monoclonal antibodies prepared against human recombinant proMMP-13. One Was for proMMP-13 and the other was for both proMMP-13 and active MMP-13. MMP-13 in samples simultaneously reacted with both solid-phase and peroxidase-labeled antibodies. The sensitivities of these EIA systems were 50pg / ml(0.83pg / well)and 80pg / ml(1.3pg / well)and linearity was obtained between 0.13 and 16ng / ml(2.1-267pg / well)as human proMMP-13 in both EIA systems, respectively. MMP-13 was detected in some serum and synovial fluids from the patients with rheumatoid arthritis(RA)and osteoarthritis(OA), but not MMP-13 level of serum from normal subjects and patients with breast cancer was below the detection limits of these assay systems. Immunoreactivity analysis showed that MMP-13 species in RA synovial fluid exists in a precursor form but not in a complex form with tissue inhibitor of metalloprotease-1(TIMP-1).

• 日本結合組織学会の論文
• 1998-03-25

著者

• Iwata Kazushi

  Biopharmaceutical Department,Fuji Chemical Industries,Ltd.

• YOSHIHARA YASUO

  Department of Orthopaedic Surgery, National Defense Medical College

• AZUMANO Isao

  Biopharmaceutical Department, Fuji Chemical Industries, Ltd.

• Knauper Vera

  Department Of Cell And Molecular Biology Strangeways Research Laboratory

• Azumano I

  Biopharmaceutical Department Fuji Chemical Industries Ltd. Takaoka Japan

• Azumano Isao

  Biopharmaceutical Department Fuji Chemical Industries Ltd.

• Otin-lopez Carlos

  Departamento De Bioquimica Biologia Molecular Facultad De Medicina Universidad De Oviedo

• Iwata K

  Biopharmaceutical Department Fuji Chemical Industries Ltd. Takaoka Japan

• Iwata Kazushi

  Biopharmaceutical Department Fuji Chemical Industries Ltd.

• Murphy Gillian

  Department Of Cell And Molecular Biology Strangeways Research Laboratory

• TAMEI Hironori

  Biopharmaceutical Department, Fuji Chemical Industries, Ltd., Takaoka, Japan

• VIZOSO Francisco

  Hospital de Jove

• Yoshihara Yasuo

  Department Of Orthopaedic Surgery National Defense Medical College

• Tamei Hironori

  Biopharmaceutical Department Fuji Chemical Industries Ltd. Takaoka Japan

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