Phorphyromonas gingivalis由来Vesicle, ProteaseおよびLPSによる線維芽細胞のCollagenase産生ならびにCytokine遺伝子の発現誘導

概要

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Secretory vesicle, protease and LPS from Porphyromonas gingivalis were studied to elucidate the abilities of cytotoxicity and induction of cytokines and type I collagenase in human fibroblast cells. The fibroblasts were killed by the vesicle in a dose dependent manner. When the cells were treated with vesicles in the presence of fetal bovine serum or α 2-macroglobulin, the damage of the cells was reduced but resulted in death under the prolonged culture. A semi-lethal amount of P. gingivalis vesicle induced secretion of type I collagenase from human fibroblasts and secretion of the enzyme was augmented by simultaneous stimulation with LPS from P. gingivalis. P. gingivalis vesicle induced the expression of mRNAs specific for interleukin-1 β (IL-1 β) , IL-6 and tumor necrosis factor α (TNF α) in the human fibroblasts with a RT-PCR analysis, While P. gingivalis protease induced the expression of mRNAs specific for IL-1 β and IL-6 in the human fibroblasts with a RT-PCR analysis. It was suggested that the vesicle from P. gingivalis is not only a part of the cell wall component but also a protease-toxin complex, and inducing trigger of inflammatory cytokines and tissue degrading enzyme in human fibroblasts. Thus the vesicle, protease and LPS from P. gingivalis are important virulence factors and may play an important role in the progress of periodontal diseases.
福岡歯科大学学会の論文
1998-03-31