電気伝導度測定法による口腔粘膜のイオン透過性
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概要
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To determine the characteristics of the absorption of substances in the oral cavity, NaCl was added to oral epithelia isolated from the frog and rat, and the induction of ion permeation and changes in potential difference across the epithelium (epithelial response) were measured. In ion permeation experiments, the tissue was sandwiched between two plastic plates with a hole to separate the mucosal surface and the muscular surface of the opposite side. After removal of ions from the tissue by perfusion of both surfaces with an iso-tonic mannitol solution, NaCl was added to the mucosal solution and ions permeated through the tissue were measured by changes in electric conductance of the muscular solution. Na ions permeated into the muscular solution were measured by a flame photometer. In the epithelial response experiments, after both surfaces were adapted to Ringer solution, NaCl was added to the mucosal solution and its induced epithelial response was measured. The results were as follows : 1. The conductivity method could measure NaCl as iondissolving in the mannitol solution to the extent of 10^<-6> or 10^<-7> M. This sensitivity was nearly the same or slightly higher than that measured by flame photometer. 2. In frog tissues, addition of NaCl to the mucosal soltion increased the electric conductance of the muscular solution due to permeation of ions through the tissue. Permeation was the greatest in the dorsal epithelium of the tongue (tongue), followed by epithelia of the soft palate, mouth floor and hard palate in descending order. 3. In the frog tongue, ion permeation induced by NaCl was greater than that induced by LiCl and less than that induced by KCl. 4. Ion permeation through the frog tongue measured by conductivity method was slightly higher than Na permeation measured by flame photometry. 5. Treatment of the frog tongue with tetracaine at 3 mM reversibly inhibited ion permeation through the tongue induced by NaCl. 6. After adaptation to Ringer solution, stimulation of the frog tissue with NaCl induced an epithelial response, associated with a decrease in resistance across the tissue. The order of the response magnitude among the tissues was similar to that observed in the conductivity method. 7. Similar to that in the frog tissues, addition of NaCl caused the electric conductance in the rat tissues to increase. However, the increases were significantly smaller than those of the frog, and the order of the permeation was as follows ; soft palate, tongue and buccal tissue. 8. In the rat tongue, the NaCl-induced ion permeation decreased with age. Based on the results obtained, ion permeation is discussed in relation to the morphological structures of the tissue. It may be concluded that the differences in ion permeation by species and region of the oral cavity were due to tissue structures suitable for differences in food intake between the two species.
- 福岡歯科大学学会の論文
- 1996-06-30
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