Porphyromonas gingivalis由来のLPSによるB細胞内チロシンリン酸化反応
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Specific Gram-negative anaerobic bacteria including Porphyromonas gingivalis have been implicated in the pathogenesis of periodontal diseases. These microorganisms possess endotoxic lipopolysaccharides (LPS) as a membrane component, the immunological potencies of which may contribute to the inflammatory reaction in periodontal tissues. It has been demonstrated that LPS isolated from some oral Gram-negative bacteria such as P.gingivalis, have a unique chemical structure. Despite the importance of the LPS-immunocompetent cell interaction, the mechanism by which LPS activates immunocompetent cells including B lymphocytes is poorly understood. Since tyrosine protein phosphorylation is thought to be part of the signal transduction mechanism that mediates later cellular responses, the molecular effect of the LPS from P.gingivalis on the tyrosine protein phosphorylation in the B lymphocytes from (LPS-responsive) C3H/HeN and (LPS-nonresponsive) C3H/HeJ strains of mice was examined in the present study. The findings indicated that P.gingivalis LPS increased tyrosine phosphorylation on the substrate proteins with apparent molecular masses of 24.8 and 26.0kDa (p24.8 and p26.0) in the B lymphocytes from C3H/HeN as well as C3H/HeJ mice, when detected under Western blot analysis by using the monoclonal anti-phosphotyrosine antibody. However, Escherichia coli LPS was found to increase tyrosine protein phosphorylation in B lymphocytes from the C3H/HeN but not from C3H/HeJ mice. Also, p24.8 and p26.0 in the B lymphocytes from C3H/HeN were similar or identical to those from C3H/HeJ mice, suggesting that the basic signaling pathways that involve tyrosine protein phosphorylation were intact in C3H/HeJ B lymphocytes. These findings suggest that through the same tyrosine-protein phosphorylation pathway as observed in C3H/HeN B lymphocytes, P.gingivalis LPS could induce the activation of C3H/HeJ B lymphocytes in which a trigger signal by E.coli LPS cannot be transduced to initiate the phosphorylation needed for gene activation that eventually leads to proliferation.
- 福岡歯科大学学会の論文
- 1995-12-31
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- Porphyromonas gingivalis由来のLPSによるB細胞内チロシンリン酸化反応
- Porphyromonas gingivalis由来のLPSによるB細胞内チロシンリン酸化反応