6-および5-ヒドロキシドーパミンのラット唾液腺の分泌機能に及ぼす作用
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概要
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No report is available, so far, on the effects of 6- and 5-hydroxydopamines (OHDA) on the secretory functions of rat submandibular (SMG) and parotid (PG) glands. This study was thus designed to determine the effects of both OHDAs on the SMG and PG at different doses used intravenously (i.v.) with and without various types of autonomic blockers and Ca ion channel ones. Two catecholamines (adrenaline and noradrenaline) were also used as partial controls. The types of protein and their amino acid compositions were elucidated by various electrophoreses and amino acid analysis. The volumes of saliva, salivary flow rates and the amounts of protein secreted, whether expressed as mg/kg or μg/mg gland/h, by the SMG and PG in response to both OHDAs and two catecholamines significantly increased with increasing doses. In contrast, the protein concentrations in both salivas elicited by the SMG and PG and amylase activities in salivas by the PG were not dependent of doses used. The values of most parameters with some exceptions in submandibular salivas in response to 6-OHDA were significantly reduced with both α - and β-adrenoceptor blockers, and atropine, whereas in response to 5-OHDA they were significantly reduced with α-adrenoceptor blockers, but not with β-ones and atropine. In contrast, the values of most parameters with some exceptions in salivas elicited by the SMG in response to 6-OHDA, but not in response to 5-0HDA, were significantly reduced with Ca ion channel blockers. No salivation in response to both OHDAs was seen in combination with both phentolamine and propranolol by the SMG, as seen by the PG with propranolol. The types of proteins in submandibular salivas in response to 6-OHDA at different doses were of the β-type, but at higher or highest doses the mixed type (αβ-type) or the α-type was observed in some rats, in contrast that in response to 5-OHDA they were of the α- and αβ-types in some rats, as seen with 6-OHDA, at all doses used. The typical α-type protein was seen in response to two catecholamines at all doses used. The β-type protein in response to both OHDAs was completely abolished by all of Ca ion channel blockers, as seen in combination with β-adrenoceptor ones with some exceptions. The α-type in response to both OHDAs was replaced to the β-type in combination with α-adrenoceptor blockers with some exceptions, whereas no change of the protein type was seen in combination with two cholinergic blockers. The concentrations of protein with an exception and amylase activities, but not other parameters, in parotid saliva in response to both OHDAs significantly increased with α-adrenergic and cholinergic antagonists. Amino acid compositions in submandibular saliva were very similar at different doses in response to both OHDAs each other, as seen in parotid saliva.
- 福岡歯科大学学会の論文
- 1995-03-31