EXPRESSION AND ACTIVITY OF MATRIX METALLOPROTEASE IN PIGMENTED VILLONODULAR SYNOVITIS

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概要

• 論文の詳細を見る

• <lntroduction>Pigmented villonodular synovitis (PVNS) is an uncommon idiopathic, proliferative synovial disease. The objective of the present study was to analyze the role of gelatinases (MMP-2 and 9), TIMP-1 and TIMP-2 in addition to MMP-1 in disease progression of PVNS, by means of immunohistochemistry, zymography, westernblotting and RT-PCR <Matenals and Methods>The study was based on synovial tissue samples obtained at surgical operation from 7 patients with a diagnosis of PVNS. Conventional immunohistochemical studies were performed using a streptavidin-biotin complex technique to detect MMPs and TIMPs. For the detection of gelatin-degrading activity, zymography was performed with conditioned medium from PVNS tissue culture. The culture media was also subjected to western blot analysis incubated with anti-MMPs and TIMPs monoclonal antibodies. Also expression of mRNA for MMPs and TIMPs in the PVNS tissues were determined with RT-PCR technique. <Results>Immunohistochemistry: PVNS of the seven cases examined comprised numerous multinucleated giant cells and mononuclear cells. MMP-1,-9 and TIMP-1 was imimunolocalized intensively and predominantly in the multinucleated giant cells. Gelatin zymography : Culture medium of PVNS from two patients expressed gelatinolytic activities at 67Kd. Western blotting: Immunoblot analysis using monoclonal antibodies to various MMPs and TIMPs revealed that MMP-1 protein was strongly detected in culture medium and MMP-9 and TIMP-1 were weakly expressed. RT-PCR: mRNA for each MMP-1,-9 and TIMP-1 were detectable.<Discussion>Our studies have demonstrated that MMP-1 and MMP-9 would be the predominant MMPs in PVNS. MMP-1 and -9 expression appeared to surpass the TIMP-1 levels by immunohistochemisiry, given that PVNS occasionally induces loss of joint spaces and degenerative arthritis, these MMPs may contribute to the bone and cartilage damage occuired in PVNS.

• 日本結合組織学会の論文

著者

• Nishida Yoshihiro

  Department Of Orthopaedic Surgery Nagoya University School Of Medicine

• Yamada Yoshihisa

  Department Of Orthopaedic Surgery Nagoya University School Of Medicine

• Nakashima Hiroatsu

  Department Of Orthopaedic Surgery Nagoya University School Of Medicine

• Tabata Izuru

  Department of Orthopaedic Surgery, Nagoya University School of Medicine

• Uchibori Mitsutoshi

  Dept. Orthop. Surg.,Pref Aichi Hosp.

• Nishida Yoshihiro

  Dept Orthop Surg., Nagoya Univ. Sch. Med.

• Tabata Izuru

  Dept Orthop Surg., Nagoya Univ. Sch. Med.

• Nakashima Hiroastu

  Dept Orthop Surg., Nagoya Univ. Sch. Med.

• Yamada Yoshihisa

  Dept Orthop Surg., Nagoya Univ. Sch. Med.

• Sugiura Hideshi

  Dept Orthop Surg., Nagoya Univ. Sch. Med.

• Sugiura Hideshi

  Department Of Orthopaedic Surgery Nagoya University School Of Medicine

• Uchibori Mitsutoshi

  Dept. Orthop. Surg. Pref Aichi Hosp.

• Tabata Izuru

  Department Of Orthopaedic Surgery Nagoya University School Of Medicine

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