AVAILABILITY OF BRACHYMORPHIC MOUSE IN BALB/C STRAIN AS AN UNDERSULFATED ANIMAL

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Brachymorphic (bm) mice are characterized by the congenital morphological disorder such as shortened limb, trunk and tail, and dome-like vertebral column (spinal curvature). This mutation is due to recessive single mutant gene (bin gene) on autosomal chromosome. Recently, it has been elucidated that the bm gene induces a point mutation of bifunctional sulfurylase kinase having both ATP-sulfurylase and adenosine-phosphosulfate kinase activities. Conclusively, decreasing synthesis of phospho-adenosine-phosphosulfate causes undersulfation of chondroitin sulfate in the cartilage matrix and results in the chondorogenesis disorder as the brachymorphic mutation in this mouse. In the present study, we tried to transfer the bm gene from original bm mice (C57BL/6-bm/bin) to BALB/c strain by outbreeding and BALB/c-bm/bm mice were histologically and histochemically examined. By histochemistry, it was found that BALB/c-bm/bm mice suffered from an undersulfation in not only connective tissues but also epithelial tissues. According to these findings, it is suggested that the BALB/c-bin/bin mice is available as an undersulfated experimental animal.
日本結合組織学会の論文