PARTIALLY FUCOSE BRANCH-REMAINING SEA CUCUMBER GLYCOSAMINOGLYCANS AND THEIR ABILITIES TO ENHANCE t-PA MEDIATED PLASMINOGEN ACTIVATION

スポンサーリンク

概要

• 論文の詳細を見る

• Many types of glycosaminoglycans (GAGs) have been reported to exhibit plasminogen potentiation ability via tissue-type plasminogen activator (t-PA) in vitro. Very recently, we demonstrated that this potentiation ability is markedly enhanced by chondroitin sulfate E (CS-E). Sea cucumber GAG (SC-GAG) was isolated from the connective tissue of the body wall of the sea cucumber Stichopus japonicus and characterized to be composed of CS-E-type core polymer with sulfated fucose branches on every disaccharide units. In order to clarify plasminogen potentiation mechanism(s) with respect to structure-activity relationship, we compared the potentiation abilities of CS-E, SC-GAG and two kinds of SC-GAG derivatives bearing different amounts of fucose branches that were prepared from intact SC-GAG by mild acid hydrolyses under different time-conditions. Almost 33% of fucose branches remained in the 3h-hydrolyzed derivative, whereas only 10% of fucose branches remained in the 6h-hydrolyzed derivative. The molecular weights of the former and the latter derivatives were calculated to be 12,000 and 10,000, respectively, while those of intact SC-GAG and CS-E were known to be 18,000 and 78,000, respectively. Chondroitinase ABC digestion analyses indicated that the contents of CS-E-type disaccharide units bearing no fucose branch were 62%, 0%, 29% and 29% for CS-E, intact SC-GAG, 3h-hydrolyzed SC-GAG and 6h-hydrolyzed SC-GAG, respectively. The plasminogen potentiation abilities via t-PA by these GAGs were evaluated as potentiation factors. At the concentration of 6.25ug/ml, potentiation factors of these GAGs calculated were about 160, 3, 44 and 7 for CS-E, intact SC-GAG, 3h-hydrolyzed SC-GAG and 6h-hydrolyzed SC-GAG, respectively. For development of t-PA-mediated plasminogen potentiation abilities by GAGs, it was found that simultaneous fulfillment of the following two factors would be needed; one is the higher contents of CS-E-type disaccharide unit bearing no fucose branch and the other is the molecular weight of the corresponding GAGs (presumably more than 11,000).

• 日本結合組織学会の論文

著者

• Kariya Yutaka

  Central Research Laboratories, Seikagaku Corporation

• Kariya Y

  Seikagaku Corp. Tokyo

• Kariya Yutaka

  Central Research Laboratories Seikagaku Corporation

• KYOGASHIMA Mamoru

  Central Research Laboratories, Seikagaku Corporation

• Sakai Tokiko

  Central Research Laboratories, Seikagaku Corporation

• Kaneko Takuji

  Central Research Laboratories, Seikagaku Corporation

• Suzuki Kiyoshi

  Central Research Laboratories, Seikagaku Corporation

• Kyogashima Mamoru

  Central Research Laboratories Seikagaku Corporation

• Suzuki Kiyoshi

  Central Research Laboratories Seikagaku Corporation

関連論文

• Effects of Heparin and Its 6-O-and 2-O-Desulfated Derivatives with Low Anticoagulant Activity on Proliferation of Human Neural Stem/Progenitor Cells(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
• Enhancement of Neurite Outgrowth-Promoting Activity by Heparin Derivatives in Sodium Chlorate-Treated Explant Cultures of Rat Central Neurons
• Effects of Dermatan Sulfate, a Heparin Cofactor II Mediated Thrombin Inhibitor, on the Endotoxin-Induced Disseminated Intravascular Coagulation Model in the Rat: Comparison With Low-Molecular Weight Heparin, Nafamostat Mesilate and Argathroban
• Enhancement of t-PA-Mediated Plasminogen Activation by Partially Defucosylated Glycosaminoglycans from the Sea Cucumber Stichopus japonicus
• PARTIALLY FUCOSE BRANCH-REMAINING SEA CUCUMBER GLYCOSAMINOGLYCANS AND THEIR ABILITIES TO ENHANCE t-PA MEDIATED PLASMINOGEN ACTIVATION
• Attenuation of glial scar formation in the injured rat brain by heparin oligosaccharides
• Characterization of Isomeric Unsulfated Glycosaminoglycan Oligosaccharides by Mass Spectrometry/Mass Spectrometry

スポンサーリンク