ラット顎下腺におけるアドレナリン受容体サブタイプ遺伝子の発現に及ぼす歯科矯正力の影響

概要

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The effects of orthodontic force on the norepinephrine (NE) concentration and gene expression of adrenoceptor (AR) subtypes in the rat submandibular glands were examined by high performance liquid chromatography (HPLC) system, reverse transcription-polymerase chain reaction (RT-PCR) and competitive-PCR during 7 days after orthodontic treatment. The findings were as follows : 1. The wet weights of the submandibular gland (SMG) significantly increased 7 days after treatment. 2. The NE levels in the SMG did not significantly change after treatment. 3. The five AR subtype genes, α_<1a>, α_<1b>, α_<2A>, β_1 and β_2ARs, were expressed in the SMG, in contrast to that any other four AR mRNAs, α_<1d>, α_<2B>, α_<2C> and β_3ARs, were not detected by RT-PCR. 4. The gene expression of β_1AR relative to a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), significantly increased 1,3 and 7 days after treatment. In contrast, the concentrations of mRNAs for α_<1a> and α_<2A>ARs were significantly reduced 3 and 7 days after treatment. These results suggest that the gene expression of α_<1a>, α_<2A> and β_1ARs, and especially β_1AR, were affected by orthodontic force, and the transcriptional regulation of various AR subtypes may be involved in enlargement of SMG caused by orthodontic force.
福岡歯科大学学会の論文
1999-09-30