骨芽細胞様細胞(ROS17/2.8)の分化・増殖に及ぼす骨代謝調節ホルモンおよび顎下腺唾液成分の影響
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It has been established that various hormones, growth factors and cytokines effect on differentiation and proliferation of osteoblast. The submandibular glands of rats can discriminate the nature of the stimulus and secrete three different electrophoretic types of proteins in response to various adrenergic agonists, as termed α, β and γ. Rat salivary cystatin can be induced in submandibular saliva by chronic treatment of β-adre- nergic agonist, isoproterenol. Recently it has been reported that the cystatins are involved in bone resorption. However, little information is available on the effects of salivary components on osteoblast. This study was thus designed to examine the effects of various bone metabolic hormones and salivary components on the differentiation and proliferation of an osteoblast-like clonal cell line, ROS 17/2.8 derived from rat osteosarcoma. Alkaline phosphatase (ALP) activity and cell numbers were used as biochemical markers of osteoblast function, that is, cell differentiation and proliferation, respectively. The results were as follows : 1. 1α, 25-dihydroxyvitamin D_3,24R, 25-dihydroxyvitamin D_3,dexamethasone, parathyroid hormone, 17 β-estradiol and testosterone increased either the ALP activity or cell number of ROS 17/2.8,whereas prostaglandin E_2 increased both of them. Furthermore, it was suggested that 1 α, 25-dihydroxyvitamin D_3 regulated the differentiation and proliferation of ROS 17/2.8 predominantly rather than other hormones. 2. Saliva of α-and β-typs and saliva treated chronically with isoproterenol (IPR-7d) contained a high percentage of salivary cystatin accelerated the differentiation of ROS 17/2.8,but inhibited the proliferation, whereas γ-type saliva increased both the differentiation and proliferation. 3. The differentiation was accelerated and the proliferation of ROS 17/2.8 was influenced by submandibular saliva containing various factors and by each fraction of α- β-and γ-types and IPR-7d saliva fractionated with ultrafiltration membranes on the basis of molecular weight differences. 4. Salivary cystatin purified from IPR-7d saliva accelerated both the differentiation and proliferation of ROS 17/2.8.
- 福岡歯科大学学会の論文
- 1996-03-31
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- 骨芽細胞様細胞(ROS17/2.8)の分化・増殖に及ぼす骨代謝調節ホルモンおよび顎下腺唾液成分の影響
- 骨芽細胞様細胞(ROS17/2.8)の分化・増殖に及ぼす骨代謝調節ホルモンおよび顎下腺唾液成分の影響