VX_2移植家兎舌癌切除後の治癒過程に関する神経組織学的研究

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The recovery of peripheral nerves in the healing process following partial resection of the tongue affected with cancer might also imply functional recovery of the tongue partially resected. The experimental tongue cancer was produced in a domestic rabbit by transplantation of VX_2-Carcinoma which pathohistologically resembles squamous cell carcinoma a representative oral cancer. After confirming the establishment of cancer, the affected site was resected and its healing process was followed neurohistologically along with observation of the unaffected tongue. Sixty two domestic rabbits each weighing approximately 2.5 kg had been kept in the Animal Center of Fukuoka Dental College with regular feeding for more than one week. They were arbitrarily divided into four groups as follows : three animals for control to observe normal tongue microscopically (group A), three for microscopic observation of cancered tongue (group B), 35 for experimental model, in which cancered tongue was resected and followed (group C), and 21 for another control, in which the unaffected tongue was similarly resected and followed (group D). Groups C and D were subdivided into seven subgroups equally and they were killed on the 10th day, 15th, 30th, 60th, 90th, 180th and 240th postoperatively. The tongue was extirpated and fixed, and serial saggital sections were made. They were stained by H-E staining, urea-silver nitrate method modified by Kitamura et al. and immunohistochemical staining method for neurofilament and S-100 protein. In group B, a degenerative change of the nerve fibers was distinctly observed in the tissue surrounding the tumor, and its degree was gradually increased with approximation to the lingual apex. The tunica subepithelium showed a stronger degree of degeneration than the muscle tissue. In an early stage following partial resection of the tongue, changes in peripheral nerves such as decrease of fibers and their irregular distribution was shown in both groups C and D, and group C showed a stronger degree of degeneration than group D. Additionally, its degree gradually increased approximati to the lingual apex, and tunica subepithelium was more greatly suffered than muscle tissue. In a later stage, peripheral nerve fibers had increased and their distribution had be come regular, however, findings of complete recovery were not observed by the 240th day postoperatively in either group C or D. In particular recovery was delayed in group C, which was thought to be caused by the distorted structure of muscle tissue affected with the tumor. Recovery was slower in the tunica subepithelium than in the muscle tissue, which consequently suggests that sensory nerves are damaged more than motor nerves.
福岡歯科大学学会の論文
1992-09-30

VX_2移植家兎舌癌切除後の治癒過程に関する神経組織学的研究

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