ブタのMyxovirus感染に関する研究 : 特にMyxovirusに対する抗体について
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概要
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Pigs have been said to play some role in an influenza epidemic. The author examinedthe antibody against myxovirus in sv.zine sera, in order to evaluate the role of pigs in thepandemic of Asian irnfluenza in 1957. During the course of these investigatioros, it wasrealized that the rnethods of measuring antibody against myxovirus presented various diffi-culties. The conditions and methods for the measuring of antibody were, therefore, examinedand the followint facts were clarified.I) There was a non-specifie HA ir,.hibitor against influenza A/Asia/57 virus. This in-hibitor seemed to be somewhat different from the inhibitors l<nown heretofore and couldnot be removed by heating at 56C for 30 min or by RDE treatrment. It could be removedby periodate treatment and the HAT test with the virus could be earried out only after theperiodate treatment of tlae sera. Unless adequate attention to the inhibitor was paid, theresults of the HAT test with the Asian influenza virus were apt to lead to a false conclusiort.The optirrnum condition for periodate treatment was found to be the rnixing of one volurrneof serum vith two volumes of M/ 100 K[O4 solution and then to neutralize the excess ofthe perioclate with glycerol after keeping the mixture overnight in the refrigerator.2) There w?as no stubborrt non-specific I-IA inhibitor in swine sera against swine in-fluenza virus and HVJ. The FIAT test of these viruses coruld be carried out easily.3) CF test of swine sera agairnsL Asian influenza, swine influenza virus and HVJ couldbe carried out using the modified Kolt"ner method (two units of cornplement, four units ofancigen, and serial dilution of scrap. Fhe CF test could also be carried out using tlte corn-plement dilution n?ethod (serial dilution of complement with constant units of antigen., andsera), but no special difference in the serodiagnostic value was found between the twornethods.4) The flocculation test of swine sera against Asian influenza virus arrd HVJ could becarried out using purified corncentrated virus
- 社団法人日本獣医学会の論文
- 1960-02-25