Protein Stretching III : Force-Extension Curves of Tethered Bovine Carbonic Anhydrase B to the Silicon Substrate under Native, Intermediate and Denaturing Conditions
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概要
- 論文の詳細を見る
Atomic force microscope (AFM) was used to measure the force-extension relationship of the globular protein, carbonic anhydrase B, having 259 amino acid residues, under native, denaturing and intermediate solution conditions. For this purpose, the protein was genetically engineered in order to endow it with -SH groups at its N- and C- termini and was fixed to the silanized surface of a silicon wafer using a covalent cross-tinker with the reactive end to -SH and with a long spacer of polyethylene glycol. The silicon nitride tip of the AFM was covered with a cross-tinker with the reactive end to -SH and was brought into contact with the protein on the silicon surface. Subsequent force curve measurements showed occasional stable bond formation, and the force extension relationship obtained from such curves showed distinct characteristics of the native, denatured and intermediate forms of carbonic anhydrase B.
- 社団法人応用物理学会の論文
- 1999-06-30
著者
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Wang T
Riken (inst. Physical And Chemical Res.) Saitama Jpn
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Wang Tong
Laboratory Of Biodynamics Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Ikai Atsushi
Laboratory Of Biodynamics Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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