除草剤glyphosateのエンドウ葉の代謝経路に及ぼす影響

概要

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Using submerged leaf disks prepared from pea plants, the effects of glyphosate on the biosynthetic pathway of pigments, shikimic acid pathway, glycolysis and TCA cycle were surveyed. Incorporation of ^14C-glucose into each fraction of chlorophylls, carotenes and xanthophylls was inhibited by the glyphosate treatment (Table 1). Incorporation of ^14C-glucose into aromatic amino acids was inhibited by the glyphosate treatment. In the treatment with a glyphosate concentration lower than 1 mM, a higher radio-activity of shikimic acid was observed and glyphosate seemed to inhibit 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase). When the concentration of glyphosate was higher than 10 mM, the incorporation of ^14C-glucose into shikimic acid was very low, presumably due to the herbicide-inhibition effect of 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (DAHP synthase) on the production of shikimic acid (Table 2). Incorporation of ^14C-glucose into the metabolites in the glycolysis and TCA cycle was not inhibited by the glyphosate treatment (Table 3). It was assumed that glyphosate mainly inhibited EPSP synthase and partially DAHP synthase and the pathways from acetyl COA to carotenoids and also from TCA cycle to δ-aminolevulinic acid, respectively, and that these inhibitions acted synergically with the herbicidal action persisting for a relatively long period of time after the application.
日本雑草学会の論文
1987-05-26