A Scanning Electron Microscopic Study of Neurons and Fibers of Human Cerebellar Cortex

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概要

• 論文の詳細を見る

• Real three-dimensional features of human cerebellar cortex were observed using scanning electron microscopy (SEM). A specimen preparation method for SEM in the present study was a t-butyl alcohol freeze-drying device at 15℃ and 160mmHg. Relatively large specimens (about 10 x 15 mm x 1 mm) of formalin-fixed human, cut with a razor blade, were rinsed in water, dehydrated in a series of ethanols, immersed in a series of t-butyl alcohol, and then placed in the new freeze-drying device. The specimens were freeze-dried without acid or alkali digestion, mounted on stubs with a diameter of 32 mm, and sputter-coated with gold. This new preparation method allowed a higher magnification of surfaces of cells and fibers of the human cerebellar cortex compared to the critical point drying method. This was valied for Purkinje cell bodies with axons, dendrites with climbing fibers and climbing fiber glomeruli, stellate neuron cell processes connected to the Purkinje cell dendrites. Lugaro cell, Golgi II cell, basket cells with axons, mossy fiber glomerulus with granule cell dendrites, ell dendrites, satellite Bergmann glial cells with processes and many microtubule-like fibrous structures on the inside of Purkinje cell dendrites are shown.

• バイオメディカル・ファジィ・システム学会の論文
• 1996-08-08

著者

• Hojo Teruyuki

  Department Of Anatomy And Anthropology School Of Medicine University Of Occupational And Environemen

• Hojo Teruyuki

  Department Of Anatomy And Anthropology School Of Medicine University Of Occupational And Environment

関連論文

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• A Scanning Electron Microscopic Study of Neurons and Fibers of Human Cerebellar Cortex

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