非細胞病原性牛ウイルス性下痢ウイルスに対する体外由来透明帯除去2・4細胞期胚と脱出胚盤胞の感受性

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概要

• 論文の詳細を見る

• To investigate the susceptibility of early bovine embryos to noncytopathogenic bovine viral diarrhea virus (NCP BVDV), 2- and 4-cell embryos produced in vitro from which zona pellucida had been removed by pronase treatment, and hatched blastocysts were exposed to 10^6 TCID_<50>/ml of NCP BVDV No. 12 strain. The virus was detected in all embryo samples immediately prior to cultivation but not in the medium. After 24-hr culture, the virus was isolated from four media and two embryo samples in four experiments in the blastocyst group, and the viral antigen was demonstrated in the cytoplasm of the embryo cells by the immunofluorescent technique. By contrast, no virus was recovered from, or viral antigen detected in samples from the 2- and 4-cell embryo group in any of the experiments, even though they were exposed to the virus after removal of the zona pellucida. These findings suggest that 2- and 4-cell embryos are unlikely to be susceptible to NCP BVDV, but that blastocysts are capable of being infected with the virus.

• 社団法人日本獣医学会の論文
• 1999-08-25

著者

• Imada Tadao

  Viral Pathogenesis Department Of Virology National Institute Of Animal Health

• TSUBOI Takamitsu

  Laboratories of Microbiological Diagnosis, Department of Systematic Diagnosis

• Tsuboi Takamitsu

  Laboratories Of Microbiological Diagnosis Department Of Systematic Diagnosis

• 坪井 孝益

  Laboratories of Microbiological Diagnosis, Department of Systematic Diagnosis

• 今田 忠男

  Viral Pathogenesis, Department of Virology, National Institute of Animal Health

関連論文

• 非細胞病原性牛ウイルス性下痢ウイルスに対する体外由来透明帯除去2・4細胞期胚と脱出胚盤胞の感受性
• Bovine Viral Diarrhea Virus Replication in Bovine Follicular Epithelial Cells Derived from Persistently Infected Heifers.

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