義歯床でのDenture plaque分布類型とFibronectinの関連性について
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概要
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The present study was desigend to investigate a distribution type of denture plaques and their structures to Fibronectin (FN) on prosthetic denture fabrication. Double staining method of the direct immunohistochemical fluorescence techique was used to investigate them. The specific antibodies against Candida albicans (Candida), aerobic and anaerobic flora obtained from denture plaque were prepared. These were labeled with fluorescein isothiocyanate by method of Hamajima and Kyogoku. And tetrarhodamine isothiocyanate-labeled antibody to FN (Cappel. Lab.) was used. The results were as follows : FN was detected in all of fortyseven samles of dentures. FN was detected conformed in statistically significance at five percent level the basal surface of the denture. Candida was observed on the upper denture especially on palatal fitting surface. statistically (significance at five percent level). Interrelationship between microganisms and FN on all of the denture surfaces was proved significantly at five percent level. A larger areas of FN and microganisms were found in the basal surface of denture than the polishing surface (p=0.05). These resalts suggested that FN is significantly related to the formation of denture pellicle.
- 日本補綴歯科学会の論文
- 1990-10-01