^3H-Thymidine標識によるアパタイト周囲組織の検索
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概要
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The objective of this study was to examine the kinetics of mesenchymal cells derived from the periosteum to apatite particles with autoradiographical evaluation using tritiated thymidine. A circular hole 4.5mm in diameter was made in each rat's parietal bone. Apatite particles were implanted on one side as the experimental group, while the other was left empty as the control group. 1.0 μCi/gram body weight of tritiated thymidine was injected intraperitoneally 1 hour before the rats were sacrificed, and 12 rats were sacrificed from 6 hours to 28 daysafter operation. On the other group, to clarify cell differentiation of labeled cells, 3.0 μCi/gram body weight of tritiated thymidine was injected intraperitoneally at 4 days after the operation and sacrificed at 28 days after the operation. Percentage of population labeling gave a highest value at 2 days after operation at each measured area, and was slightly higher in the experimental group than in the control. The labeled cells were mainly seen close to some cells but away from the bone surface and the apatite particles. From the results of long term labeling, the labeled cells were seen in the newly formed bone around the apatite particles and in the area where there was no bone formation, the labeled cells were seen in the surface of the apatite particles. From these results, it is suggested that bone formation at the surface of the apatite particles originated from the osteoprogenitor cell in the mesenchymal cells which is derived from the periosteum differentiated to osteoblasts.
- 社団法人日本補綴歯科学会の論文
- 1988-08-01
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関連論文
- リン酸カルシウム系セラミックスに関する実験的研究 : 骨形成タンパク質-ハイドロキシアパタイト-アテロコラーゲン複合体の骨膜下移植について
- ^3H-Thymidine標識によるアパタイト周囲組織の検索
- 2-5-2. アパタイト周囲組織の組織計量学的および^3H-thymidine標識による経時的変化の観察(第76回日本補綴歯科学会学術大会講演要旨)