家兎白血球の解糖能,アルカリフオスフアターゼ活性値およびアシツドフオスフアターゼ活性値に及ぼすエックス線全身照射の影響について

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Because thrombocytes and prostata influence alkaline phosphatase and acid phosphatase activities, neither blood nor serum specimens are adequate for the examination of these activities. In order to avoid these undesirable influences, the auther separated the leucocytes using nonwettable glasswares treated with Beckman's Desicote, and exmined the effects of total-body X-ray exposure on the glycolysis, alkaline phosphatase and acid phosphatase activities of the separated leucocytes of the rabbit. Methods and metarials: Animals: albino male rabbits each weighing 2∼2.5kg. X-ray irradiation: 200kv 15mA through the filter consisting of 0.9 mm Cu and 0.5 mm Al. foucus-animal distance 70 cm, 400r (19.6 r/min.)on the A group (15 rabbits) 100r ( : ) on the B group ( : ) 50r ( : ) on the C group ( : ) 12.5r ( : ) on the D group ( : ) non irradiation in the control group (10 rabbits) Sampling of blood specimens: sampled from the vein of animals using glasswares which was treated with Desicote and put with heparin. sampling time 1.) 1 hr. after exposure, 2.) 5 hr. after exposure, 3.) 3 days after exposure, 4.) 5 days after exposure, 5.) 10 days after exposure, Leucocytes separation: Kagami and Yamada's method (cf. table 1.) Glucose estimation: Somogji's method, Phosphatase estimation: Shinowara-Jones-Reinhart's method, Exprimental results: Effect on the glycolysis activity: 50 and 12.5 r exposure: as shown in Fig. 1, 400 and 100 r exposure: as shown in Fig. 2, Effect on the alkaline phosphatase activity;, 50 and 12.5 r exposure: as shown in Fig. 3, 400 and 100 r exposure: as shown in Fig. 4. Effect on the acid phosphatase activity: 50 and 12.5 r exposure: as shown in Fig. 5, 400 and 100 r exposure: as shown in Fig. 6. Conclusion: 1. In cases of 400 and 100 r X-ray exposures, glycolysis, alkaline phosphatase and acid phosphatase activities decreased markedly within 5 houres and showed the lowest value in the specimens of 5 hours and 3 days after exposure, and the value increased as the period after exposure increased. 2. In cases of 50 and 12.5 r X-ray exposures, on the contrary, these activities showed the highest value in the specimens of 1 hour or 5 hours after exposure, and the value decreased as the period after exposure increased. 3. Changes in these activities were detectable even with 12.5 r exposure. 4. These findings show that phosphatase activities should be subjected to examination studying the effect of X-ray.
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家兎白血球の解糖能,アルカリフオスフアターゼ活性値およびアシツドフオスフアターゼ活性値に及ぼすエックス線全身照射の影響について

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