An alternative method for mitotic stimulation in fish cytogenetics

概要

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The in vitro mitotic stimulation, using Saccharomyces cerevisae suspension at glucose-enriched medium, has been widely adopted as a basic methodology for the increasing of mitotic index in fish species, due to its reduced costs, easy performance and good efficacy. However, when used in small, fragile or stressed specimens, it frequently causes a high mortality level. In the present work, the marine fish species Abudefduf saxatilis, Stegastes fuscus, and S. pictus were used to evaluate the efficacy of the pharmaceutical compound Munolan (Allergan Frumtost), an association of bacterial and fungal antigens, as mitotic agent. Comparative analyses among different treatments using Saccharomyces cerevisae, Munolan or a combination of both were performed. The best efficacy was obtained by the isolated use of Munolan, coupled with a mortality level decreasing. The results showed the efficiency of the method, encouraging its use for routine cytogenetical studies in fish.
財団法人染色体学会の論文
2001-00-00