2. Whole mounted chromosomes and kinetochores of pollen mother cells and tapetal cells : Three-dimensional ultrastructure of in situ chromosomes and kinetochores of Tradescantia reflexa anther cells by scanning electron microscopy
スポンサーリンク
概要
- 論文の詳細を見る
Whole mounted meiotic and somatic chromosomes and kinetochores of Tradescantia reflexa were demonstrated stereoscopically by SEM using the heating method in an acetic acid solution. Entire chromosomes and kinetochores at each dividing stage in pollen mother cells and tapetal cells frorn anthers could clearly be observed in situ being released from cytoplasrnic material. In comparing the meiotic chromosomes and kinetochores with the somatic ones in detail, differences were revealed in chromatin organization and how kinetochores appeared. Chromosomes which were composed of chromatin fibers of 30-50 nm in diameter showed a highly compacted and coiling or solenoidal appearance in meiotic chromosomes but a loosely organized fibrous appearance in somatic chromosomes under the high-resolution SEM. Furthermore, a small hole or pit was frequently recognized in the center of the arm end not only in bivalent chromosomes but also in separated chromatids, and the diameter of metaphase I chromosomes was almost twice the size of the chromatids in the second meiotic stage. Therefore, it seemed that the central hole or pit corresponded to the position of the coil axis and the two chromatids in bivalents or univalents formed a double helical configuration. Each chromatid occurring in the second meiotic division forming a solenoid structure was probably made by single coiling. New profiles of kinetochores were demonstrated on the centromeric region of meiotic chromosomes appearing as "crown-like" protrusions from the side and irregular "ring like" structures from the front. These characteristic structures might be the first three-dimensional SEM images of the "cup" of plant kinetochore structure, previously described as "ball and cup".
- 財団法人染色体学会の論文
著者
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Inaga S
Fac. Med. Tottori Univ.
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Inaga Sumire
Faculty Of Medicine Tottori University
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Inaga Sumire
Department Of Anatomy Faculty Of Medicine Tottori University
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Naguro T
Fac. Med. Tottori Univ.
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Naguro Tomonori
Department Of Anatomy Faculty Of Medicine Tottori University
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IINO Akihiro
Department of Anatomy, Faculty of Medicine, Tottori University
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Kameie Toshio
Department of Anatomy, Faculty of Medicine, Tottori University
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Iino A
Emeritus Prof. Tottori Univ.
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Iino Akihiro
Department Of Anatomy Faculty Of Medicine Tottori University
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Kameie T
Fac. Med. Tottori Univ.
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